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parameter tm pge 2 assay kit  (R&D Systems)


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    Structured Review

    R&D Systems parameter tm pge 2 assay kit
    Celecoxib inhibits <t>PGE</t> <t>2</t> production in D17 canine osteosarcoma cells in a dose-dependent manner. D17 cells were treated with celecoxib (0, 60, and 80 μM) for 48 h. PGE 2 concentrations in the culture medium were quantified by ELISA. Data represent mean ± SD. * p < 0.01 versus vehicle control by one-way ANOVA followed by Dunnett’s post hoc test.
    Parameter Tm Pge 2 Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 969 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/parameter+tm+pge+2+assay+kit/pmc13030460-91-7-13?v=R%26D+Systems
    Average 96 stars, based on 969 article reviews
    parameter tm pge 2 assay kit - by Bioz Stars, 2026-07
    96/100 stars

    Images

    1) Product Images from "Celecoxib Inhibits Vasculogenic Mimicry and Induces Apoptosis in the D17 Canine Osteosarcoma Cell Line via the COX-2/PGE2 Signaling Axis"

    Article Title: Celecoxib Inhibits Vasculogenic Mimicry and Induces Apoptosis in the D17 Canine Osteosarcoma Cell Line via the COX-2/PGE2 Signaling Axis

    Journal: Veterinary Sciences

    doi: 10.3390/vetsci13030288

    Celecoxib inhibits PGE 2 production in D17 canine osteosarcoma cells in a dose-dependent manner. D17 cells were treated with celecoxib (0, 60, and 80 μM) for 48 h. PGE 2 concentrations in the culture medium were quantified by ELISA. Data represent mean ± SD. * p < 0.01 versus vehicle control by one-way ANOVA followed by Dunnett’s post hoc test.
    Figure Legend Snippet: Celecoxib inhibits PGE 2 production in D17 canine osteosarcoma cells in a dose-dependent manner. D17 cells were treated with celecoxib (0, 60, and 80 μM) for 48 h. PGE 2 concentrations in the culture medium were quantified by ELISA. Data represent mean ± SD. * p < 0.01 versus vehicle control by one-way ANOVA followed by Dunnett’s post hoc test.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Control

    Exogenous PGE 2 rescues celecoxib-inhibited vasculogenic mimicry formation in D17 canine osteosarcoma cells. ( a ) Representative phase-contrast images of VM formation in D17 cells treated with vehicle control, 80 μM celecoxib, or 80 μM celecoxib + 100 ng/mL PGE2 at 7 h and 24 h post-seeding on Matrigel. ( b ) Quantitative analysis of VM network parameters (master junctions, master segments, meshes) at 7 h using ImageJ software. ( c ) Quantitative analysis of VM network parameters at 24 h. Magnification, ×40. Data represent mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle control by one-way ANOVA followed by Dunnett’s post hoc test.
    Figure Legend Snippet: Exogenous PGE 2 rescues celecoxib-inhibited vasculogenic mimicry formation in D17 canine osteosarcoma cells. ( a ) Representative phase-contrast images of VM formation in D17 cells treated with vehicle control, 80 μM celecoxib, or 80 μM celecoxib + 100 ng/mL PGE2 at 7 h and 24 h post-seeding on Matrigel. ( b ) Quantitative analysis of VM network parameters (master junctions, master segments, meshes) at 7 h using ImageJ software. ( c ) Quantitative analysis of VM network parameters at 24 h. Magnification, ×40. Data represent mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle control by one-way ANOVA followed by Dunnett’s post hoc test.

    Techniques Used: Control, Software



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    Image Search Results


    Celecoxib inhibits PGE 2 production in D17 canine osteosarcoma cells in a dose-dependent manner. D17 cells were treated with celecoxib (0, 60, and 80 μM) for 48 h. PGE 2 concentrations in the culture medium were quantified by ELISA. Data represent mean ± SD. * p < 0.01 versus vehicle control by one-way ANOVA followed by Dunnett’s post hoc test.

    Journal: Veterinary Sciences

    Article Title: Celecoxib Inhibits Vasculogenic Mimicry and Induces Apoptosis in the D17 Canine Osteosarcoma Cell Line via the COX-2/PGE2 Signaling Axis

    doi: 10.3390/vetsci13030288

    Figure Lengend Snippet: Celecoxib inhibits PGE 2 production in D17 canine osteosarcoma cells in a dose-dependent manner. D17 cells were treated with celecoxib (0, 60, and 80 μM) for 48 h. PGE 2 concentrations in the culture medium were quantified by ELISA. Data represent mean ± SD. * p < 0.01 versus vehicle control by one-way ANOVA followed by Dunnett’s post hoc test.

    Article Snippet: PGE 2 levels were quantified using the Parameter TM PGE 2 assay kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol.

    Techniques: Enzyme-linked Immunosorbent Assay, Control

    Exogenous PGE 2 rescues celecoxib-inhibited vasculogenic mimicry formation in D17 canine osteosarcoma cells. ( a ) Representative phase-contrast images of VM formation in D17 cells treated with vehicle control, 80 μM celecoxib, or 80 μM celecoxib + 100 ng/mL PGE2 at 7 h and 24 h post-seeding on Matrigel. ( b ) Quantitative analysis of VM network parameters (master junctions, master segments, meshes) at 7 h using ImageJ software. ( c ) Quantitative analysis of VM network parameters at 24 h. Magnification, ×40. Data represent mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle control by one-way ANOVA followed by Dunnett’s post hoc test.

    Journal: Veterinary Sciences

    Article Title: Celecoxib Inhibits Vasculogenic Mimicry and Induces Apoptosis in the D17 Canine Osteosarcoma Cell Line via the COX-2/PGE2 Signaling Axis

    doi: 10.3390/vetsci13030288

    Figure Lengend Snippet: Exogenous PGE 2 rescues celecoxib-inhibited vasculogenic mimicry formation in D17 canine osteosarcoma cells. ( a ) Representative phase-contrast images of VM formation in D17 cells treated with vehicle control, 80 μM celecoxib, or 80 μM celecoxib + 100 ng/mL PGE2 at 7 h and 24 h post-seeding on Matrigel. ( b ) Quantitative analysis of VM network parameters (master junctions, master segments, meshes) at 7 h using ImageJ software. ( c ) Quantitative analysis of VM network parameters at 24 h. Magnification, ×40. Data represent mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle control by one-way ANOVA followed by Dunnett’s post hoc test.

    Article Snippet: PGE 2 levels were quantified using the Parameter TM PGE 2 assay kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol.

    Techniques: Control, Software